Biological photonic crystals revealed by multimodality nonlinear microscopy

被引：0

作者：

Sun, CK ^{[1
]}

Chu, SW ^{[1
]}

Chen, IH ^{[1
]}

Lin, BL ^{[1
]}

Cheng, PC ^{[1
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Electroopt Engn, Taipei 10617, Taiwan

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES II | 2002年 / 4620卷

关键词：

photonic crystal; second harmonic generation; third harmonic generation; laser scanning microscopy; multiphoton microscopy;

D O I：

10.1117/12.491288

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A novel multi-modality nonlinear microscopy reveals highly optically-active biophotonic crystal structures in living cells. Numerous biological structures, including stacked membranes and arranged protein structures are highly organized in optical scale and are found to exhibit strong optical activities through second-harmonic-generation (SHG) interactions, behaving similar to man-made photonic crystals. The microscopic technology developed is based on a combination of imaging modalities including not only SHG, but also third-harmonic-generation and multi-photon-fluorescence. With no energy deposition during harmonic generation processes, the demonstrated highly-penetrative yet non-invasive microscopy is useful for investigating the dynamics of structure-function relationship at the molecular and subcellular levels and is ideal for studying living cells that require minimal or no preparation.

引用

页码：166 / 174

页数：9