Expression of Clostridium perfringens Alpha-Epsilon Soluble Fusion Toxin Gene in Escherichia coli and its Immunogenicity in Mice

被引：0
|
作者：
Song, Xiaohui [1 ]
Sun, Yu [1 ]
Xiao, Ying [2 ]
Liu, Yuliang [1 ]
Wang, Ruinan [1 ]
Jiang, Fei [1 ]
Zhang, Cunrui [1 ]
Yang, Lin [1 ]
Wang, Chuanbin [1 ]
机构：
[1] China Anim Dis Control Ctr CADC, Beijing 102600, Peoples R China
[2] Chongqing Anim Dis Control Ctr, Chongqing 401120, Peoples R China
来源：
INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY | 2019年 / 22卷 / 03期
关键词：
Clostridium perfringens; Alpha-epsilon toxin gene; Immunogenicity; Soluble expression and purification; Soluble fusion protein; ENTEROTOXEMIA;
D O I：
10.17957/IJAB/15.1084
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
In this study, a set of molecular biotechnologies were used to efficiently express and obtain soluble fusion protein of alphaepsilon-toxin (alpha-epsilon-toxin) derived from Clostridium perfringens in Escherichia coli. We have successfully obtained a soluble fusion protein of alpha-epsilon-toxin for the first time with efficient expression in E. coli system by conducting codon optimization, removing the signal peptide, selecting sequences of higher hydrophilicity and antigenicity, removing the lethal gene and optimizing the expression conditions. Noticeable rise of antibody level was detected in the serum of mice after immunization with the expressed protein. The immunized mice got protected against C. perfringens type A, B, C and D with the survival rate of 100,90,85 and 100%, respectively. (C) 2019 Friends Science Publishers
引用
收藏
页码：443 / 448
页数：6
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