Crystallization and preliminary X-ray crystallographic studies of trichosanthin delta C7

被引：1
|
作者：
Li, X
Ding, Y
Too, H
Wang, Z
Liu, Y
Dong, Y
Shaw, P
Rao, Z
机构：
[1] Tsing Hua Univ, Sch Life Sci & Engn, MOE Lab Prot Sci, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, Struct Biol Lab, Beijing 100084, Peoples R China
[3] Chinese Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
[4] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China
[5] Xian Jiaotong Univ, Coll Med, Xian 710061, Peoples R China
来源：
PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 03期
关键词：
trichosanthin delta C7; ribosome-inactivating proteins; antigenicity; crystallization;
D O I：
10.2174/0929866023408742
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) which possesses rRNA N-glycosidase activity. TCS has various pharmacological properties. It is possible to reduce the antigenicity of TCS by deleting up to seven C-terminal residues of TCS (TCS-C7) with minimal effect on its activity [1]. TCS-C7 has been crystallized and the crystal diffracted to 1.8 Angstrom. It belongs to space group P2(1), with unit-cell parameters a = 71.6Angstrom, b = 74.4Angstrom, c = 87.6Angstrom, beta = 97.0degrees. It is given that there are four molecules per asymmetric unit.
引用
收藏
页码：269 / 273
页数：5
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