GT1-7 Cell-Based cytoxicity screening assay on 96-well microplates as a platform for the safety assessment of genetically modified Gerbera hybrida extracts

被引：2

作者：

Fallarero, Adyary ^{[1
]}

Ainasoja, Miia ^{[2
]}

Sandberg, Malena ^{[1
]}

Teeri, Teemu H. ^{[2
]}

Vuorela, Pia M. ^{[1
]}

机构：

[1] Abo Akad Univ, Dept Biochem & Pharm, Fac Math & Nat Sci, FIN-20520 Turku, Finland

[2] Univ Helsinki, Fac Agr Forestry, Dept Appl Biol, Gerbera Lab, Helsinki, Finland

来源：

DRUG AND CHEMICAL TOXICOLOGY | 2009年 / 32卷 / 02期

基金：

芬兰科学院;

关键词：

Screening; GT1-7; cells; natural products; gerbera hybrida; INDUCED OXIDATIVE STRESS; IN-VITRO MODELS; MADS-BOX GENE; NEURONS; ASTERACEAE; LINES; NEUROTOXICITY; CYTOTOXICITY; TOXICITY; PATTERNS;

D O I：

10.1080/01480540802593857

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this investigation, a GT1-7 cell-based cytotoxicity screening assay in 96-well microplates was set up. The assay, using propidium iodide fluorescence, was proven to be reliable, with good quality (Z'=0.51) and low plate-to-plate and day-to-day variations. Further on, a library containing extracts from 227 genetic modification (GM) Gerbera hybrida and 42 Gerbera varieties was screened; however, no differences between them were found. Based on these findings, we propose the use of the current assay within the first-tier screening studies of large collections. Also, these results provide valuable information for GM Gerbera risk-assessment purposes and offer a model for the toxicity cell-based screening of GM crops.

引用

页码：120 / 127

页数：8