Angiotensin-(1-7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats

被引:6
|
作者
Liu, Juan [1 ]
Liu, Yan [1 ]
Pan, Wenyou [1 ]
Li, Yongsheng [1 ]
机构
[1] Nanjing Med Univ, Dept Rheumatol, Affiliated Huaian Peoples Hosp 1, 1 Huanghe West Rd, Huaian 223300, Peoples R China
关键词
Collagen-induced arthritis; Rat; Rheumatism; Inflammatory cytokines; Oxidative stress; RHEUMATOID-ARTHRITIS; RECEPTOR; MODEL; SYSTEM; INFLAMMATION;
D O I
10.1007/s00726-020-02935-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study was designed to investigate the anti-rheumatic effects and the mechanism of angiotensin (Ang)-(1-7) in rat models with collagen-induced arthritis (CIA). The CIA model was established using male Wistar rats by intradermal injection of bovine collagen-II in complete Freund's adjuvant at the base of the tail. The levels of angiotensin converting enzyme 2 (ACE2)/Ang-(1-7)/Mas receptor (MasR) were reduced in CIA rats. The attenuation of paw swelling and arthritis scores and improvement of indexes of spleen and thymus were done by Ang-(1-7) injection in CIA rats. The increased levels of inflammatory cytokines, such as interleukin (IL)-1 beta, IL-6, tumor necrosis factor (TNF)-alpha, and interferon (IFN)-gamma in the serum and hind paw were blocked by Ang-(1-7) administration. In addition, enhanced NADPH oxidase (Nox) activity, increased levels of superoxide anions and malondialdehyde (MDA), and weakened superoxide dismutase (SOD) activity, were all reversed by treatment with Ang-(1-7). Nox1 overexpression reversed the suppressing effects of Ang-(1-7) on paw swelling and arthritis scores in CIA rats. The Ang-(1-7)-induced improvement in spleen and thymus indexes in CIA rats was abolished by Nox1 overexpression. Nox1 overexpression reversed the inhibitory effects of Ang-(1-7) by increasing IL-1 beta, IL-6, TNF-alpha, and IFN-gamma levels in the serum and hind paw of CIA rats. These results demonstrated that Nox1 increased the oxidative stress in arthritis, and Ang-(1-7) improved rheumatism in arthritis via inhibiting oxidative stress.
引用
收藏
页码:171 / 181
页数:11
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