AN ENRICHED STABLE-ISOTOPE APPROACH TO DETERMINE THE GILL-ZINC BINDING PROPERTIES OF JUVENILE RAINBOW TROUT (ONCORHYNCHUS MYKISS) DURING ACUTE ZINC EXPOSURES IN HARD AND SOFT WATERS

被引：11

作者：

Todd, Andrew S. ^{[1
]}

Brinkman, Stephen ^{[2
]}

Wolf, Ruth E. ^{[1
]}

Lamothe, Paul J. ^{[1
]}

Smith, Kathleen S. ^{[1
]}

Ranville, James F. ^{[3
]}

机构：

[1] US Geol Survey, Denver, CO 80225 USA

[2] Colorado Div Wildlife, Ft Collins, CO 80526 USA

[3] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA

来源：

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2009年 / 28卷 / 06期

关键词：

Zinc; Stable isotopes; Gills; Rainbow trout; Biotic ligand model; BIOTIC LIGAND MODEL; CHRONIC WATERBORNE; SALMO-GAIRDNERI; CHRONIC TOXICITY; DAPHNIA-MAGNA; CD UPTAKE; ACCUMULATION; CADMIUM; COPPER; ACCLIMATION;

D O I：

10.1897/08-252.1

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The objective of the present study was to employ an enriched stable-isotope approach to characterize Zn uptake in the gills of rainbow trout (Oncorhynchus mykiss) during acute Zn exposures in hard water (similar to 140 mg/L as CaCO(3)) and soft water (similar to 30 mg/L as CaCO(3)). Juvenile rainbow trout were acclimated to the test hardnesses and then exposed for up to 72 h in static exposures to a range of Zn concentrations in hard water (0-1,000 mu g/L) and soft water (0-250 mu g/L). To facilitate detection of new gill Zn from endogenous gill Zn, the exposure media was significantly enriched with (67)Zn stable isotope (89.60% vs 4.1% natural abundance). Additionally, acute Zn toxicity thresholds (96-h median lethal concentration [LC50]) were determined experimentally through traditional, flow-through toxicity tests in hard water (580 mu g/L) and soft water (110 mu g/L). Following short-term (<= 3 h) exposures, significant differences in gill accumulation of Zn between hard and soft water treatments were observed at the three common concentrations (75, 150, and 250 mu g/L), with soft water gills accumulating more Zn than hard water gills. Short-term gill Zn accumulation at hard and soft water LC50s (45-min median lethal accumulation) was similar (0.27 and 0.20 mu g/g wet wt, respectively). Finally, comparison of experimental gill Zn accumulation, with accumulation predicted by the biotic ligand model, demonstrated that model output reflected short-term (< 1 h) experimental gill Zn accumulation and predicted observed differences in accumulation between hard and soft water rainbow trout gills. Our results indicate that measurable differences exist in short-term gill Zn accumulation following acclimation and exposure in different water hardnesses and that short-term Zn accumulation appears to be predictive of Zn acute toxicity thresholds (96-h LC50s).

引用

页码：1233 / 1243

页数：11