Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia

被引：18

作者：

Kralova, Tereza ^{[1
]}

Cegan, Radim ^{[1
]}

Kubat, Zdenek ^{[1
]}

Vrana, Jan ^{[3
]}

Vyskot, Boris ^{[1
]}

Vogel, Ivan ^{[1
,2
]}

Kejnovsky, Eduard ^{[1
,2
]}

Hobza, Roman ^{[1
,3
]}

机构：

[1] Inst Biophys ASCR, Dept Plant Dev Genet, CZ-61200 Brno, Czech Republic

[2] Masaryk Univ, CEITEC Cent European Inst Technol, Lab Genome Dynam, Brno, Czech Republic

[3] Inst Expt Bot ASCR, Ctr Reg Hana Biotechnol & Agr Res, Ctr Plant Struct & Funct Genom, Olomouc, Czech Republic

来源：

CYTOGENETIC AND GENOME RESEARCH | 2014年 / 143卷 / 1-3期

关键词：

Microdissection; Sex chromosomes; Silene latifolia (white campion); Retrotransposons; GENOME SIZE; Y-CHROMOSOME; DROSOPHILA-MELANOGASTER; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; SEQUENCE ALIGNMENT; HIGH-THROUGHPUT; EVOLUTION; PLANT; RICE;

D O I：

10.1159/000362142

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel

引用

页码：87 / 95

页数：9

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