HER1-4 protein concentrations in normal breast tissue from breast cancer patients are expressed by the same profile as in the malignant tissue

被引:9
|
作者
Olsen, Dorte Aa. [1 ]
Ostergaard, Birthe [2 ]
Bokmand, Susanne [3 ]
Wamberg, Peter A. [3 ]
Jakobsen, Erik H. [4 ]
Jakobsen, Anders [4 ]
Brandslund, Ivan [1 ,5 ]
机构
[1] Vejle Cty Hosp, Dept Clin Biochem, DK-7100 Vejle, Denmark
[2] Vejle Cty Hosp, Dept Pathol, DK-7100 Vejle, Denmark
[3] Vejle Cty Hosp, Breast Canc Ctr, DK-7100 Vejle, Denmark
[4] Vejle Cty Hosp, Dept Oncol, DK-7100 Vejle, Denmark
[5] Univ So Denmark, Fac Hlth Sci, Odense, Denmark
关键词
breast cancer; breast tissues; epidermal growth factor receptor (EGFR); enzyme linked immunosorbent assay (ELISA); epidermal growth factor receptor; ErbB; human epidermal growth factor receptor (HER); GROWTH-FACTOR RECEPTOR; IN-SITU HYBRIDIZATION; TYROSINE KINASES; CONSERVING SURGERY; PROGNOSTIC VALUE; ERBB-RECEPTORS; EGFR FAMILY; HER-2/NEU; SURVIVAL; HER2;
D O I
10.1515/CCLM.2009.214
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: The epidermal growth factor receptor HER2 is overexpressed or amplified in 25%-30% of patients with breast cancer. The mechanism behind HER2 amplification is unknown, but may be a pathophysiological phenomenon caused by continuous stimulation and activation of the HER1-4 system. We have mapped the protein concentrations of HER1-4 in breast cancer tissue, autologous reference tissue, normal breast tissue and serum samples, to see whether non-cancer cells from these patients express a protein profile indicating general activation. Methods: Tissue samples from malignant and adjacent normal breast tissue (autologous reference tissue) were collected from 118 women consecutively admitted for surgical treatment of breast cancer. In addition, 26 samples of normal breast tissue were collected from healthy women having breast reduction surgery. The tissue samples were homogenized and the proteins extracted. The tissue and serum concentrations of HER1-4 were determined quantitatively using a commercially available enzyme linked immunosorbent assay (ELISA) method. Results: HER1 was down regulated in cancer tissue when compared to autologous reference tissue (p=8x10(-6)), while HER2 (p<10(-7)) and HER3 (p=3x10(-5)) were up regulated. Comparing autologous reference tissue with normal tissue showed down regulation of HER1 (p=0.122) and up regulation of HER2 (p=10(-6)), HER3 (p<10(-7)) and HER4 (p<10(-7)). Furthermore, we observed that correlations between the receptor combinations HER1-2, HER1-3 and HER1-4 were maintained from normal breast tissue to autologous reference breast tissue, but were lost in cancer tissue. Conclusions: We suggest that these findings indicate that breast cancer is a systemic disease where the HER1-4 system in autologous reference tissue is continuously activated, thus favoring the subsequent development of cancer. Clin Chem Lab Med 2009;47:977-84.
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页码:977 / 984
页数:8
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