Nanoparticles Grafted by ATP Aptamer: Preparation and Application in Chemiluminescence Enzyme Analysis

Oleic acid-modified Fe3O4 nanoparticles was prepared and dopamine hydrochloride was chosen to amino-modify these nanoparticles. The Fe3O4 nanoparticles have good water-dispersible, and were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) spectroscopy, vibrating sample magnetometer and ultravioletvisible spectroscopy (UV-Vis) spectra. Then, amino-modified adenosine triphosphate (ATP) aptamer was grafted onto Fe3O4 nanoparticles, and the amount of ATP was detected with luciferase chemiluminescence method. Meanwhile, the amount of ATP of lactic acid bacteria in commercially yogurt was tested, which had high sensitivity and good reproducibility. The results showed that these functionalized-Fe3O4 nanoparticles could act as good dispersion, easy separation carrier, which indicated uniform particle size, good stability, strong magnetism and excellent binding ability with ATP aptamer. The development of Fe3O4 nanoparticles largely expanded its application in the field of analysis.