Histopathology of Bipolar Radiofrequency Ablation in the Human Atrium

Background. The Cox maze IV operation has become the preferred surgical treatment for atrial fibrillation, as it is associated with less morbidity and complexity than the Cox maze III procedure, yet is still highly effective. Numerous studies have been conducted in animals to examine the histopathology of this operation on the heart but studies on human hearts that have undergone the Cox maze IV operation have not been performed. Methods. We report the histopathologic findings in 3 patients from whom tissue was available for histologic study. In 2 patients it was obtained at autopsy within a month after undergoing a Cox maze IV operation, and in the remaining patient, atrial tissue was obtained immediately after ablation. Results. The lesions were clearly visible on the atria at day 6 and day 18. Microscopic examination showed that the hearts were in different stages of healing. We also found that, compared with animal models, human myocardium had significant preexisting underlying damage with myocyte hypertrophy and fibrosis. Although most of the ablative lesions were transmural, not all spanned from the epicardium to the endocardium. The chronic changes present in these hearts may have prevented transmurality by impeding energy delivery from fully penetrating the tissue. Conclusions. The atrial myocardial substrate studied in experimental conditions is markedly different from the human hearts that frequently express histopathologic changes secondary to the underlying disease process. That may prevent creating true transmural lesions and impact final efficacy of the procedure. (C) 2016 by The Society of Thoracic Surgeons