Fuel injection parameters play an important role in diesel engine performance for obtaining proper combustion. The performance and emission characteristics of diesel engine depend on many parameters. The aim of the work is to experimentally investigate the effect of number of injection nozzle holes on the performance and emission characteristics of the compression ignition engines operated with soya ethyl ester blended with mineral diesel. This paper discusses the results of investigation carried out on a single cylinder, four stroke, and direct injection diesel engine. Number of nozzle holes are considered for the analysis were single holed, three holed, five holed, seven holed and nine holed injector nozzle. All tests with different fuels were conducted at 1500 rpm with varying load conditions on the engine. Comparative measures of Brake thermal efficiency (BTE), Specific fuel consumption (SFC), Mechanical efficiency, Carbon monoxide emission (CO), Hydrocarbon emission (HC) and Oxides of nitrogen (NO x) has been presented and discussed. Engine performance in terms of brake thermal efficiency and lower emission with different blends of biodiesel (B10, B30, B50) were observed. The performance characteristics of soya ethyl ester blends were found to be closer to diesel and the emission parameters of the engine is improved significantly. From the experimental investigation it is found that the five holed injector nozzle with B30 blend produces significant increase in brake thermal efficiency with decrease in specific fuel consumption. The result showed for emission characteristics such as CO decreases with increase in nozzle hole and gradually increases at seven holed nozzle and similarly NO x emission and HC emission is comparably less in five holed injector nozzle and in B30 blend when compared to other nozzles and blends. The reduction in emission and specific fuel consumption and increase in brake thermal efficiency made the soya ethyl ester a suitable alternative fuel for diesel engine at hinder compression ratio and which helps in reducing the air pollution.