The aim of this study was to determine the energetic factors of middle-distance running performance in junior elite runners according to gender and by using measurements from on-track performances. Fifteen elite runners (8 males and 7 females) were investigated by means of an incremental test and an all-out run over 600 m performed with a 2-d interval. We calculated (1) the aerobic maximal power (E-r max aero, in Wkg(-1)), including VO2 max and the delay of attainment of VO2 max in the 600 m run; (2) the anaerobic power (E-r max anaero), i.e., the oxygen deficit (J kg(-1)) divided by the duration of the 600 m run. Despite the difference in race duration (87+/-3 vs. 102+/-2s), the 600 m run was made at the same relative value of the velocity associated with VO2 max (VVO2 (max)) in males and females (121.6+/-7 vs. 120+/-8% VO2 max, p=0.7). E-r max aero explained most of the variance in the performance (the personal best performed 8 weeks later) between genders: 65 and 79% over 800 m (T-800) and 1,500 m (T-1,T-500). For females, E-r max aero explained most of the variance of T-1,T-500 (r(2) = 0.66), and E-r (max anaero) improved this prediction (r(2) = 0.84). No energetic factor predicted the performance on 800 m run in males. In elite junior athletes, the energetic model with individual data measured over an all-out 600 m performed on a track, provides an explanation for most of the variance in middle-distance running performances between genders. The distinction between aerobic power and anaerobic power allowed an improvement in the prediction of middle-distance running performances.