Optimization of label-free nano LC-MS/MS analysis of the placental proteome

被引：5
|
作者：
Luyten, Leen J. [1 ,2 ]
Dieu, Marc [1 ,3 ]
Demazy, Catherine [1 ,3 ]
Fransolet, Maude [1 ,3 ]
Nawrot, Tim S. [2 ,4 ]
Renard, Patricia [1 ,3 ]
Debacq-Chainiaux, Florence [1 ]
机构：
[1] Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium
[2] Hasselt Univ UHasselt, Ctr Environm Sci, Diepenbeek, Belgium
[3] Univ Namur UNamur, Mass Spectrometry Facil, MaSUN, Namur, Belgium
[4] Leuven Univ KULeuven, Dept Publ Hlth & Primary Care, Occupat & Environm Med, Leuven, Belgium
来源：
PLACENTA | 2020年 / 101卷
关键词：
Placenta; Proteomics; Label-free nano LC-MS/MS; MEMBRANE; PROTEINS;
D O I：
10.1016/j.placenta.2020.09.013
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.
引用
收藏
页码：159 / 162
页数：4
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