A novel monoclonal antibody to N-myristoyl glycine moiety found a new N-myristoylated HIV-1 p28(gag) protein in HIV-1-infected cells

A novel monoclonal antibody was raised against a synthetic N-myristoyl glycine that is characteristic of all N-myristoylated proteins. The immunoreaction suppressed in the presence of hemocyanin as well as albumin conjugated with N-myristoyl glycine and other N-myristoyl glycyl peptides, while underivatized and myristoyl amino acid proteins or various fatty acids other than myristic acid exerted no effect. The antibody specifically reacted with N-myristoylated pp60(c-src) in human colon adenocarcinoma cells, N-myristoylated pp60(v-src) in Rous sarcoma virus-infected cells, and N-myristoylated Gag precursor protein Pr55(gag) in HIV-1-producing cells. Furthermore, the antibody immunoreacted with a new N-myristoylated p28(gag) derived from HIV-1 gag protein. The antibody is shown to be a very useful tool for identification of N-myristoylated proteins. (C) 1996 Academic Press, Inc.