Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe

被引：41
|
作者：
Ohi, R [1 ]
Feoktistova, A [1 ]
Gould, KL [1 ]
机构：
[1] VANDERBILT UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT CELL BIOL, NASHVILLE, TN 37232 USA
来源：
GENE | 1996年 / 174卷 / 02期
关键词：
fission yeast; plasmids; cloning; gene disruption; recombinant DNA;
D O I：
10.1016/0378-1119(96)00085-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The construction of vectors for use in Schizosaccharomyces pombe using the his3(+) gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
引用
收藏
页码：315 / 318
页数：4
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