Diagnostic value of the isotype-specific ELISA for the detection of antibodies against swine vesicular disease virus (SVDV)

被引：0
|
作者：
Niedbalski, W [1 ]
机构：
[1] Natl Inst Vet Res, Dept Foot & Mouth Dis, PL-98220 Zdunska Wola, Poland
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2002年 / 46卷 / 01期
关键词：
pig; SVD; isotype-specific ELISA; singleton reactors;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A trapping-indirect ELISA was used to determine the isotype of the antibody against SVDV in pigs experimentally infected with SVDVPOL/73. The first antibodies were detectable as early as 4 days after experimental infection. Up to the 9th day, demonstrable antibodies were exclusively of the IgM class. At this day the IgG developed and remained as a plateaux level up to the 28th d.p.i. The similar results were obtained in pigs immunized with experimental vaccine prepared from SVDV antigen. The use of trapping-indirect ELISA allowed to significantly reduce the number of non-specific positive results (singleton reactors) obtained by virus neutralization test and MAC-ELISA method.
引用
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页码：37 / 44
页数：8
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