Development, Characterization Optimization, and Assessment of Curcumin-Loaded Bioactive Self-Nanoemulsifying Formulations and Their Inhibitory Effects on Human Breast Cancer MCF-7 Cells

被引:23
|
作者
Kazi, Mohsin [1 ]
A. Nasr, Fahd [2 ]
Noman, Omar [2 ]
Alharbi, Abdulrahman [1 ]
Alqahtani, Mohammed S. [1 ]
Alanazi, Fars K. [1 ]
机构
[1] King Saud Univ, Coll Pharm, Dept Pharmaceut, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Med Aromat & Poisonous Plants Res Ctr, Coll Pharm, Riyadh 11451, Saudi Arabia
关键词
bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs); solubility improvement; curcumin; thymoquinone; antioxidant activity; drug precipitation; MTT assay; DRUG-DELIVERY SYSTEMS; LIPID-BASED FORMULATIONS; ORAL BIOAVAILABILITY ENHANCEMENT; IN-VITRO; PARTICLE-SIZE; THYMOQUINONE; PRECIPITATION; DISPERSION; DESIGN; SMEDDS;
D O I
10.3390/pharmaceutics12111107
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Curcumin (CUR) is an attractive polyphenol for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties. Poor solubility in water and sensitivity against sunlight are the most challenging characteristics in the development of CUR for clinical use. The aim is to develop oral lipid-based bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs) for curcumin as a candidate for cancer therapy. Bio-SNEDDSs containing black seed oil, medium-chain mono- and diglycerides, and surfactants were prepared as CUR delivery vehicles. The morphology, droplet size, physical stability, encapsulation efficiency, risk of precipitation, lipid digestion, antioxidant activity, and antimicrobial activity were evaluated for the representative formulations. Finally, an MTT assay was performed on MCF-7 cells to determine the cytotoxic effect of the different formulations. The results showed lower droplet size (28.53 nm) and higher drug-loading (CUR 20 mg, thymoquinone 1.2 mg) for the representative Bio-SNEDDS (black seed oil/Imwitor 988/KolliphorEL (35/15/50) % w/w), along with a transparent appearance upon aqueous dilution. The dynamic dispersion and in-vitro lipolysis data proved that the Bio-SNEDDS was able to keep the CUR in a solubilized form in the gastrointestinal tract. From the antioxidant and antimicrobial studies, it was suggested that the Bio-SNEDDS had the highest activity for disease control. The MTT assay showed that the representative Bio-SNEDDS treatment led to a reduction of cell viability of MCF-7 cells compared to pure CUR and conventional SNEDDSs. A Bio-SNEDDS with elevated entrapment efficiency, antioxidant/antimicrobial activities, and an antiproliferative effect could be the best anticancer drug candidate for potential oral delivery.
引用
收藏
页码:1 / 19
页数:19
相关论文
共 50 条
  • [1] A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line
    Mirakabad, Fatemeh Sadat Tabatabaei
    Akbarzadeh, Abolfazl
    Milani, Morteza
    Zarghami, Nosratollah
    Taheri-Anganeh, Mortaza
    Zeighamian, Vahideh
    Badrzadeh, Fariba
    Rahmati-Yamchi, Mohammad
    ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2016, 44 (01) : 423 - 430
  • [2] Preparation and characterization of (-)-Epigallocatechin-3-gallate (EGCG)-loaded nanoparticles and their inhibitory effects on Human breast cancer MCF-7 cells
    Zeng, Liang
    Yan, Jingna
    Luo, Liyong
    Ma, Mengjun
    Zhu, Huiqun
    SCIENTIFIC REPORTS, 2017, 7
  • [3] Preparation and characterization of (−)-Epigallocatechin-3-gallate (EGCG)-loaded nanoparticles and their inhibitory effects on Human breast cancer MCF-7 cells
    Liang Zeng
    Jingna Yan
    Liyong Luo
    Mengjun Ma
    Huiqun Zhu
    Scientific Reports, 7
  • [4] Effects of Quercetin-Loaded Nanoparticles on MCF-7 Human Breast Cancer Cells
    Niazvand, Firoozeh
    Orazizadeh, Mahmoud
    Khorsandi, Layasadat
    Abbaspour, Mohammadreza
    Mansouri, Esrafil
    Khodadadi, Ali
    MEDICINA-LITHUANIA, 2019, 55 (04):
  • [5] In Vitro Efficacy of Curcumin-Loaded Amine-Functionalized Mesoporous Silica Nanoparticles against MCF-7 Breast Cancer Cells
    Mohebian, Zahra
    Babazadeh, Mirzaagha
    Zarghami, Nosratollah
    ADVANCED PHARMACEUTICAL BULLETIN, 2023, 13 (02) : 317 - 327
  • [6] Inhibitory actions of genistein in human breast cancer (MCF-7) cells
    Chen, WF
    Huang, MH
    Tzang, CH
    Yang, MS
    Wong, MS
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2003, 1638 (02): : 187 - 196
  • [7] Inhibitory effects of salidroside on MCF-7 breast cancer cells in vivo
    Sun, An-Qi
    Ju, Xiu-Lian
    JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2020, 48 (11)
  • [8] Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
    Han, Xiao
    Deng, Shan
    Wang, Ning
    Liu, Yafei
    Yang, Xingbin
    FOOD & NUTRITION RESEARCH, 2016, 60
  • [9] Optimized semisolid self-nanoemulsifying system based on glyceryl behenate: A potential nanoplatform for enhancing antitumor activity of raloxifene hydrochloride in MCF-7 human breast cancer cells
    Badr-Eldin, Shaimaa M.
    Aldawsari, Hibah M.
    Ahmed, Osama A. A.
    Alhakamy, Nabil A.
    Neamatallah, Thikryat
    Okbazghi, Solomon Z.
    Fahmy, Usama A.
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2021, 600
  • [10] Assessment of cranberry bush on MCF-7 human breast cancer cells
    Kaan, Dilek
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2022, 59 (10): : 985 - 997