Deep transcriptome sequencing reveals differences in global gene expression between normal and pale, soft, and exudative turkey meat

被引:7
|
作者
Malila, Y. [1 ]
Carr, K. M. [2 ]
Ernst, C. W. [3 ]
Velleman, S. G. [4 ]
Reed, K. M. [5 ]
Strasburg, G. M. [1 ]
机构
[1] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA
[2] Michigan State Univ, Res Technol Support Facil, E Lansing, MI 48824 USA
[3] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA
[4] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA
[5] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
基金
美国食品与农业研究所;
关键词
deep transcriptome sequencing; immunoblot; pale soft and exudative; pyruvate dehydrogenase kinase isozyme 4; turkey; SARCOPLASMIC-RETICULUM; ALPHA-RYR; MUSCLE; IDENTIFICATION; MECHANISMS; SUBUNIT; RELEASE; QUALITY; GAMMA; PORK;
D O I
10.2527/jas.2013-7293
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Previous studies from our laboratory suggested that differential expression of genes between normal and pale, soft, and exudative (PSE) turkey is associated with development of the PSE syndrome. However, a detailed understanding of molecular mechanisms responsible for the development of this meat defect remains unclear. The objective of this study was to extend and complement our previous work by using deep transcriptome RNA sequence analysis to compare the respective transcriptome profiles and identify molecular mechanisms responsible for the etiology of PSE turkey meat. Turkey breasts (n = 43) were previously classified as normal or PSE using marinade uptake as an indicator of quality (high = normal; low = PSE). Total RNA from breast muscle samples with the highest (n = 4) and lowest (n = 4) marinade uptake were isolated and sequenced using the Illumina GA(IIx) platform. The results indicated differential expression of 494 loci (false discovery rate < 0.05). Changes in gene expression were confirmed using quantitative real-time PCR. Pathway analysis of differentially expressed genes suggested abnormalities of calcium homeostasis and signaling pathways regulating actin cytoskeleton structure as well as carbohydrate metabolism and energy production in PSE samples. Dysregulation of postmortem glucose oxidation in PSE turkey was suggested by both dramatic downregulation of pyruvate dehydrogenase kinase, isozyme 4 (PDK4) mRNA, the most downregulated gene, and a decrease in the protein product (P = 0.0007) as determined by immunoblot analysis. These results support the hypothesis that differential expression of several genes and their protein products contribute to development of PSE turkey.
引用
收藏
页码:1250 / 1260
页数:11
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