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Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro
被引:8
|作者:
El-Sanea, Amro M.
[1
]
Abdoon, Ahmed Sabry S.
[1
]
Kandil, Omaima M.
[1
]
El-Toukhy, Nahed E.
[2
]
El-maaty, Amal M. Abo
[1
]
Ahmed, Hodallah H.
[2
]
机构:
[1] Natl Res Ctr, Vet Res Div, Dept Anim Reprod & Artificial Inseminat, Tahrir St, Cairo 12622, Egypt
[2] Cairo Univ, Dept Anim Physiol, Fac Vet Med, Cairo 12211, Egypt
关键词:
antioxidants;
buffalo oocytes;
developmental competence;
in vitro embryo production;
O-2;
tension;
EMBRYO QUALITY;
BOVINE OOCYTES;
ASCORBIC-ACID;
CUMULUS CELLS;
MELATONIN;
MATURATION;
IMPROVES;
SUPPLEMENTATION;
FERTILIZATION;
EXPRESSION;
D O I:
10.14202/vetworld.2021.78-84
中图分类号:
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号:
0905 ;
摘要:
Aim: Oxidative stress (OS) is one of the major disruptors of oocyte developmental competence, which appears due to the imbalance between the production and neutralization of reactive oxygen species (ROS). Materials and Methods: In Experiment 1, buffalo oocytes were in vitro matured, fertilized, and cultured at 38.5 degrees C under 5% CO2 + 20% O-2 in standard CO2 incubator (OS) or under 5% 2 + 5% CO2 + 90% N-2 (Multi-gas incubator, low O-2). In Experiment 2, buffalo cumulus oocytes complexes (COCs) were matured in Basic maturation medium (BMM) composed of TCM199+ 10% FCS+ 10 mu g/ml FSH+ 50 mu g/ml gentamicin (control group) or in BMM supplemented with 50 mu M ascoibic acid (ascoibic acid group) or 3.0 mM glutathione (glutathione group) or 10(-5) M melatonin (melatonin group) and cultured at 38.5 degrees C under 20% O-2 for 24 h. Matured buffalo oocytes in control, ascoibic acid, or melatonin groups were fertilized and zygotes were cultured for 8 days under the same conditions. Results: In both experiments. maturation, cleavage, and blastocyst rates were recorded. Results showed that culture of buffalo oocytes under low O-2 (5% O-2) significantly increased maturation, cleavage, and blastocyst rates (p<0.05). Meanwhile, under 20% O-2, addition of 10(-5) M melatonin or 50 mu M ascoibic acid to in vitro maturation (IVM) medium significantly improved cumulus cell expansion, nuclear maturation rates of buffalo oocytes (p<0.05), and increased cleavage and blastocyst rates (p<0.05). Conclusion: About 5% O-2 is the optimum condition for in vitro production of buffalo embryos, and addition of 10(-5) M melatonin to IVM medium for oocytes cultured under 20% O-2 could alleviate the adverse effect of high oxygen tension and increased embryo yield.
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页码:78 / 84
页数:7
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