Synthesis of regenerated bacterial cellulose-zinc oxide nanocomposite films for biomedical applications

Regenerated bacterial cellulose (RBC) composites with zinc-oxide nanoparticles (ZnO) were prepared using a new strategy for enhanced biomedical applications of BC. Powdered BC was dissolved in N-methylmorpholine-N-oxide, and different concentrations of ZnO nanoparticles were mixed into the BC solution. RBC, RBC-ZnO1 (1 % ZnO) and ZnO-RBC2 (2 % ZnO) nanocomposite films were prepared by casting the solutions through an applicator. FE-SEM images confirmed the structural features and impregnation of the RBC films by nanoparticles. XRD analysis indicated the presence of specific peaks for RBC and ZnO in the composites. The RBC nanocomposites were found to have greatly enhanced thermal, mechanical and biological properties. Specifically, the degradation temperatures were improved from 334 A degrees C for RBC to 339 and 344 A degrees C for RBC-ZnO1 and RBC-ZnO2, respectively. The mechanical strength and Young's modulus of the composites were also higher than those of pure RBC. The greatly improved antibacterial properties of the RBC-ZnO nanocomposites are the most striking feature of the present study. The bacterial growth inhibition measured for the RBC was zero, but reached up to 34 and 41 mm for RBC-ZnO1 and RBC-ZnO2, respectively. In addition to their antibacterial properties, the RBC-ZnO nanocomposites were found to be nontoxic and biocompatible with impressive cell adhesion capabilities. These RBC-ZnO nanocomposites can be used for different biomedical applications and have the potential for use in bioelectroanalysis.