Oxidative damage to lipids and proteins induced by aflatoxin B1 in fish (Labeo rohita)-protective role of Amrita Bindu

In the present study, fish (Labeo rohita) were treated with a single intraperitoneal administration of aflatoxin B-1 (AFB(1))(100 mug/100g BW). The resultant oxidative damage to lipids (measured as conjugated diene and lipid peroxidation (LPO)) and proteins (protein carbonyl) in liver, kidney and brain at the end of 3rd and 6th day was assessed. Our results showed that AFB(1) induced a significant increase in conjugated diene formation and LPO notonly in liver but also in kidney and brain. A parallel increase in protein carbonyl level was observed in these tissues. When 1: 1 mixture of 20% solution of Amrita Bindu (a salt-spice-herbal mixture based on Indian system of medicine) was co-administered along with 100 mug AFB(1), the AFB(1) induced increase in conjugated diene, LPO and protein carbonylation were minimised to a greater extent. These results led to conclusion that (i) AFB(1) not only induces oxidative damage to the primary target organ-liverin L. rohita, but also in kidney and brain, (ii) co-administration of Amrita Bindu confers protection to lipids and protein against the AFB(1) induced oxidative damage in all the three tissues. (C) 2004 Published by Elsevier B.V.