Emission Anisotropy of Fluorescein Covalently Linked to Oligonucleotides

被引：4
|
作者：
Blokhin, A. P. [1 ]
Kvach, M. V. [2 ]
Povedailo, V. A. [1 ]
Shmanai, V. V. [2 ]
Yakovlev, D. L. [1 ]
机构：
[1] Natl Acad Sci Belarus, BI Stepanov Inst Phys, 68 Nezavisimost Ave, Minsk 220072, BELARUS
[2] Natl Acad Sci Belarus, Inst Phys Organ Chem, Minsk, BELARUS
来源：
JOURNAL OF APPLIED SPECTROSCOPY | 2017年 / 84卷 / 01期
关键词：
oligonucleotides; fluorescent label; fluorescence anisotropy; rotational depolarization; internal rotation; EXTENDED DIFFUSION; INTERNAL-ROTATION; MOLECULES;
D O I：
10.1007/s10812-017-0420-8
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Rotational depolarization of fluorescence from fluorescein covalently linked to oligonucleotides was studied. Fluorescence anisotropy of two nucleic acids was measured as a function of the temperature-to-viscosity ratio in buffer solutions with various glycerin concentrations. It was shown that the experimental results could be satisfactorily explained by a diffusion model of an elongated molecular top with internal rotation. It was found that the coefficient of internal rotational diffusion in all instances was 1.5-2 times greater than that for rotation around the oligonucleotide axis.
引用
收藏
页码：19 / 24
页数：6
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