Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody

被引：30

作者：

Cho, Ki Joon ^{[1
,2
]}

Schepens, Bert ^{[1
,2
]}

Moonens, Kristof ^{[3
,4
]}

Deng, Lei ^{[1
,2
]}

Fiers, Walter ^{[1
,2
]}

Remaut, Han ^{[3
,4
]}

Saelens, Xavier ^{[1
,2
]}

机构：

[1] VIB, Ctr Med Biotechnol, Ghent, Belgium

[2] Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium

[3] VIB, Struct & Mol Microbiol, Struct Biol Res Ctr, Brussels, Belgium

[4] Vrije Univ Brussel, Struct Biol Brussels, Brussels, Belgium

来源：

JOURNAL OF VIROLOGY | 2016年 / 90卷 / 01期

关键词：

MONOCLONAL-ANTIBODY; NEUTRALIZING ANTIBODY; HUMAN RHINOVIRUS; PEPTIDE ANTIGEN; M2; PROTEIN; COMPLEX; ECTODOMAIN; EPITOPE; HIV-1; SITE;

D O I：

10.1128/JVI.02105-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the crystal structure of the M2 ectodomain (M2e) in complex with a monoclonal antibody that binds the amino terminus of M2. M2e extends into the antibody binding site to form an N-terminal beta-turn near the bottom of the paratope. This M2e folding differs significantly from that of M2e in complex with an antibody that binds another part of M2e. This suggests that M2e can adopt at least two conformations that can elicit protective antibodies.

引用

页码：611 / 615

页数：5

共 50 条

[1] Structure of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus in Complex with a Protective Monoclonal Antibody
Cho, Ki Joon
Schepens, Bert
Seok, Jong Hyeon
Kim, Sella
Roose, Kenny
Lee, Ji-Hye
Gallardo, Rodrigo
Van Hamme, Evelien
Schymkowitz, Joost
Rousseau, Frederic
Fiers, Walter
Saelens, Xavier
Kim, Kyung Hyun
JOURNAL OF VIROLOGY, 2015, 89 (07) : 3700 - 3711
[2] Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus
Ozawa, Tatsuhiko
Jin, Aishun
Tajiri, Kazuto
Takemoto, Masaya
Okuda, Tomoko
Shiraki, Kimiyasu
Kishi, Hiroyuki
Muraguchi, Atsushi
ANTIVIRAL RESEARCH, 2011, 91 (03) : 283 - 287
[3] INTERACTION OF THE AMINO-TERMINUS OF AN INFLUENZA-VIRUS PROTEIN WITH MITOCHONDRIA
WOODFIN, BM
KAZIM, AL
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 306 (02) : 427 - 430
[4] Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective
De Filette, Marina
Ysenbaert, Tine
Roose, Kenny
Schotsaert, Michael
Roels, Stefan
Goossens, Els
Schepens, Bert
Fiers, Walter
Saelens, Xavier
JOURNAL OF GENERAL VIROLOGY, 2011, 92 : 301 - 306
[5] Introduction of a temperature-sensitive phenotype into influenza A/WSN/33 virus by altering the basic amino acid domain of influenza virus matrix protein
Liu, T
Ye, ZP
JOURNAL OF VIROLOGY, 2004, 78 (18) : 9585 - 9591
[6] Influenza A virus infection engenders a poor antibody response against the ectodomain of matrix protein 2
Feng, JingQi
Zhang, Manxin
Mozdzanowska, Krystyna
Zharikova, Darya
Hoff, Henry
Wunner, William
Couch, Robert B.
Gerhard, Walter
VIROLOGY JOURNAL, 2006, 3 (1)
[7] Influenza A virus infection engenders a poor antibody response against the ectodomain of matrix protein 2
JingQi Feng
Manxin Zhang
Krystyna Mozdzanowska
Darya Zharikova
Henry Hoff
William Wunner
Robert B Couch
Walter Gerhard
Virology Journal, 3
[8] Crystal structure of the extracellular domain of human myelin protein zero
Liu, Zhigang
Wang, Yong
Yedidi, Ravikiran S.
Brunzelle, Joseph S.
Kovari, Iulia A.
Sohi, Jasloveleen
Kamholz, John
Kovari, Ladislau C.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2012, 80 (01) : 307 - 313
[9] The native structure of the assembled matrix protein 1 of influenza A virus
Peukes, Julia
Xiong, Xiaoli
Erlendsson, Simon
Qu, Kun
Wan, William
Calder, Leslie J.
Schraidt, Oliver
Kummer, Susann
Freund, Stefan M., V
Krausslich, Hans-Georg
Briggs, John A. G.
NATURE, 2020, 587 (7834) : 495 - +
[10] The native structure of the assembled matrix protein 1 of influenza A virus
Julia Peukes
Xiaoli Xiong
Simon Erlendsson
Kun Qu
William Wan
Leslie J. Calder
Oliver Schraidt
Susann Kummer
Stefan M. V. Freund
Hans-Georg Kräusslich
John A. G. Briggs
Nature, 2020, 587 : 495 - 498

← 1 2 3 4 5 →