Characterization of tomato apical stunt viroid isolated from a 24-year old seed lot of Capsicum annuum

被引：7

作者：

Verhoeven, J. Th. J. ^{[1
]}

Koenraadt, H. M. S. ^{[2
]}

Westenberg, M. ^{[1
]}

Roenhorst, J. W. ^{[1
]}

机构：

[1] Natl Plant Protect Org Netherlands, POB 9102, NL-6700 HC Wageningen, Netherlands

[2] Naktuinbouw, POB 40, NL-2370 AA Roelofarendsveen, Netherlands

来源：

ARCHIVES OF VIROLOGY | 2017年 / 162卷 / 06期

关键词：

TIME RT-PCR; SPINDLE; POSPIVIROIDS; VALIDATION; RNA;

D O I：

10.1007/s00705-017-3277-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tomato apical stunt viroid (TASVd) has been identified in a 24-year old seed lot of Capsicum annuum produced in Taiwan. It is the first finding of TASVd in this plant species. The isolate could be discriminated from all reported isolates of TASVd based on its nucleotide sequence, which showed only 94.8% identity with the most related genotype of TASVd. This discrimination was substantiated by phylogenetic analysis. Inoculation of a RNA extract of contaminated seeds to healthy pepper plants showed that the infectivity of the viroid had remained over time. Nevertheless, no transmission to seedlings was observed.

引用

页码：1741 / 1744

页数：4