Analysis of isoniazid-resistant transposon mutants of Mycobacterium smegmatis

被引：15
|
作者：
BillmanJacobe, H
Sloan, J
Coppel, RL
机构：
[1] Department of Microbiology, Monash University, Clayton, Vic.
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 144卷 / 01期
关键词：
mycobacteria; isoniazid; katG; catalase/peroxidase;
D O I：
10.1016/0378-1097(96)00336-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The emergence of multidrug-resistant tuberculosis has renewed interest in the study of drug resistance in mycobacteria with the objective of improved chemotherapy. The genetic basis of isoniazid resistance in a model mycobacterium was studied. Eleven isoniazid-resistant mutants of Mycobacterium smegmatis were created using transposon mutagenesis. Genetic and enzymatic characterisation of the mutants showed that katG, encoding T-catalase, was inactivated. The nucleotide sequence of M. smegmatis katG was determined and the mutation sites mapped demonstrating that both the amino and carboxyl halves of T-catalase an important for enzymatic activity.
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页码：47 / 52
页数：6
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