Monoclonal antibodies and an indirect ELISA for detection of psychrotrophic bacteria in refrigerated milk

被引：11
|
作者：
Gutierrez, R [1 ]
Gonzalez, I [1 ]
Garcia, T [1 ]
Carrera, E [1 ]
Sanz, B [1 ]
Hernandez, PE [1 ]
Martin, R [1 ]
机构：
[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT NUTR & BROMATOL 3,E-28040 MADRID,SPAIN
来源：
JOURNAL OF FOOD PROTECTION | 1997年 / 60卷 / 01期
关键词：
refrigerated milk; Pseudomonas fluorescens; psychrotrophic bacteria; monoclonal antibodies; enzyme-linked immunosorbent assay (ELISA);
D O I：
10.4315/0362-028X-60.1.23
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an indirect ELISA format for the detection of Pseudomonas spp. and related psychrotrophic bacteria in refrigerated milk. The immunorecognition of monoclonal antibodies adsorbed to bacteria bound to the wells of a microtiter plate was performed with rabbit anti-mouse immunoglobulins conjugated to horseradish peroxidase. Subsequent enzymic conversion of the substrate resulted in distinct absorbance differences when assaying milk samples containing psychrotrophic bacteria in the range 10(5) to 10(9) CFU ml(-1). The detection threshold for the ELISA assay developed in this work is 10(5) CFU ml(-1).
引用
收藏
页码：23 / 27
页数：5
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