Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR

被引：0
|
作者：
Jagtap, Santosh C. [1 ]
Jagtap, Chandrakant B. [1 ]
Kumar, Pradeep [1 ]
Srivastava, R. B. [1 ]
机构：
[1] Minist Def, Dept Marine Biotechnol, Naval Mat Res Lab, Bombay 421506, Maharashtra, India
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 2009年 / 55卷 / 02期
关键词：
Bacillus sphaericus; mosquito larvicidal; multiplex colony PCR; btx/bin and mtx toxin genes; POLYMERASE CHAIN-REACTION;
D O I：
10.1139/W08-113
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.
引用
收藏
页码：207 / 209
页数：3
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