A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens

被引：11

作者：

Petersdorf, Sabine ^{[1
]}

Herma, Miriam ^{[1
]}

Rosenblatt, Meike ^{[1
]}

Layer, Franziska ^{[2
]}

Henrich, Birgit ^{[1
]}

机构：

[1] Univ Dusseldorf, Univ Clin, Inst Med Microbiol & Hosp Hyg, Dusseldorf, Germany

[2] Natl Ctr Staphylococci & Enterococci, Dept Infect Dis, Robert Koch Inst, Wernigerode, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 12期

关键词：

TIME PCR ASSAY; GENE; BACTEREMIA; HOMOLOG; DENMARK; SAMPLES; SPAIN;

D O I：

10.1128/JCM.02328-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) screening using real-time PCR has decreased in sensitivity due to the emergence of variant staphylococcal cassette chromosome mec element (SCCmec) types. We have designed and validated a novel SCCmec XI primer, which enables for the first time the rapid detection of mecC-harboring MRSA directly from nasopharyngeal swabs without prior cultivation.

引用

页码：3938 / 3941

页数：4

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