Aptamer-Based Label-Free Immunosensors Using Carbon Nanotube Field-Effect Transistors

被引：100

作者：

Maehashi, Kenzo ^{[1
]}

Matsumoto, Kazuhiko ^{[1
]}

Takamura, Yuzuru ^{[2
]}

Tamiya, Eiichi ^{[3
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Research, Osaka 5670047, Japan

[2] JAIST, Sch Mat Sci, Nomi City, Ishikawa 9231292, Japan

[3] Osaka Univ, Grad Sch Engn, Dept Appl Phys, Osaka 5650871, Japan

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 11期

关键词：

Aptamers; Label-free immunosensors; Carbon nanotubes; Field-effect transistors; Debye length; Immunoglobulin E; Dissociation constant; IN-VITRO; SINGLE; BINDING; IGE; ANTIBODIES; BIOSENSOR; SELECTION; TRANSPORT; ANTIGENS; LIGANDS;

D O I：

10.1002/elan.200804552

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aptamer-based label-free immunosensors were fabricated for immunoglobulin E (IgE) detection using carbon nanotube field-effect transistors (CNTFETs). Aptamers are artificial oligonucleotides and thus are smaller than the Debye length. After aptamers were covalently immobilized on CNT channels. the electrical properties of the CNTFETs were monitored in real time. Introduction of IgE at various concentrations Caused a sharp decrease in the source-drain current and gradual saturation at lower Currents. From the measurement of the dependence of CNTFET electrical properties on IgE concentration, the dissociation constant between aptamer and IgE reactions was estimated to be 1.9 x 10(-9) M using the Langmuir adsorption isotherm.

引用

页码：1285 / 1290

页数：6

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