Binding specificity of avian heat shock protein 108

被引:3
|
作者
Weiner, KXB [1 ]
Hayes, GR [1 ]
Lucas, JJ [1 ]
机构
[1] SUNY HLTH SCI CTR,DEPT BIOCHEM & MOL BIOL,SYRACUSE,NY 13210
关键词
D O I
10.1006/bbrc.1997.7593
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chicken heat shock protein 108 (HSP108), the avian homolog of GRP94, was originally isolated from hen oviduct and binds Fe-ovotransferrin (Fe-OTf). The liver is also a rich source, and liver membranes bind Fe-OTf with a K-D of 1.7 x 10(-7) RI, a value similar to oviduct membranes. A competition assay, based on the binding of I-125-Fe-OTf to, liver membranes, was utilized to examine the binding specificity of HSP108. Ovalbumin and avidin competed effectively, with K-D's of 1.8 x 10(-7) M and 1.4 x 10(-7) RI, respectively. Iron-free OTf bound with a 10-fold higher K-D. Egg white lysozyme, chicken IgG, human transferrin, rabbit muscle actin, and porcine insulin do not bind. Neither do denatured ovalbumin or ovalbumin tryptic peptides. Thus, the binding activity of HSP108 is not restricted to Fe-OTf, nor is it universal. (C) 1997 Academic Press.
引用
收藏
页码:673 / 676
页数:4
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