Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection

被引:10
|
作者
Wang, Chenguang [1 ,2 ]
Wang, Wei [3 ]
Xu, Yi [1 ]
Zhao, Xiaoshuang [4 ]
Li, Shuainan [1 ,2 ]
Qian, Qiuling [1 ,2 ]
Mi, Xianqiang [1 ,2 ,4 ,5 ,6 ]
机构
[1] Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Shanghai Univ Med & Hlth Sci, Shanghai Pudong New Dist Zhoupu Hosp, Affiliated Zhoupu Hosp, Shanghai 201318, Peoples R China
[4] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Funct Mat Informat, Shanghai 200050, Peoples R China
[5] CAS Ctr Excellence Superconducting Elect CENSE, Shanghai 200050, Peoples R China
[6] Chinese Acad Sci, Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou 310024, Peoples R China
关键词
tetrahedral DNA framework; gold nanoparticles; hybridization chain reaction; cell-free DNA; electrochemical biosenors; HYBRIDIZATION CHAIN-REACTION; NANOSTRUCTURE; AMPLIFICATION; INTERFACE; PLATFORM;
D O I
10.3390/nano12040666
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Tumor-associated cell-free DNA (cfDNA) is a dynamic biomarker for genetic analysis, early diagnosis and clinical treatment of cancers. However, its detection has limitations because of its low abundance in blood or other complex bodily fluids. Herein, we developed an ultrasensitive cfDNA electrochemical biosensor (E-cfDNA sensor) based on tetrahedral DNA framework (TDF)-modified gold nanoparticles (Au NPs) with an interface for cfDNA detection. By accurately controlling the numbers of base pairs on each DNA framework, three types of TDFs were programmed: 26 base pairs of TDF; 17 base pairs of TDF; and 7 base pairs of TDF (TDF-26, TDF-16 and TDF-7, respectively). We also combined the TDF with hybridization chain reaction (HCR) to achieve signal amplification. Under optimal conditions, we detected the breast cancer susceptibility gene 1 (BRCA-1), a representative cfDNA closely related to breast cancer. An ultra-low detection limit of 1 aM with a linear range from 1 aM to 1 pM by TDF-26 was obtained, which was superior to the existing methods. Each type of TDF has excellent discrimination ability, which can distinguish single mismatch. More significantly, we also detected BRCA-1 in mimic serum samples, demonstrating that the E-cfDNA sensor has potential use in clinical research.
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页数:11
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