O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B

被引：1
|
作者：
Di Guida, Rossella [1 ]
Casillo, Angela [1 ]
Corsaro, Maria Michela [1 ]
机构：
[1] Univ Naples Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia 4, I-80126 Naples, Italy
来源：
CARBOHYDRATE RESEARCH | 2020年 / 497卷 / 497期
关键词：
Lipopolysaccharide; NMR; Psychrotolerant; Pseudomonas; Gulopyranuronamide; PSYCHROPHILIC BACTERIUM; CAPSULAR POLYSACCHARIDE; LIPOPOLYSACCHARIDE; LIPOOLIGOSACCHARIDE; CHAIN; SUGARS;
D O I：
10.1016/j.carres.2020.108125
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: -> 4)-alpha-L-GulpNA-c30AcAN-(1 -> 3)-beta-D-QuipNAc-(1 ->.
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页数：5
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