Gold-Nanoparticle-Conjugated Citrate Inhibits Tumor Necrosis Factor-' Expression via Suppression of Nuclear Factor Kappa B (NF-KB) Activation in Breast Cancer Cells

One of the leading causes of death worldwide is cancer. Excessive production of tumor necrosis factor (TNF)-a is known to activate nuclear transcription factor (NF)-KB, which plays a lethal role in the onset of multiple disorders including cancer. In this study, we aimed to determine the therapeutic role of novel gold nanoparticles conjugated with citrate (AuNPs-CIT) on the elevated expression of TNF-a in breast cancer cells. AuNPs-CIT were synthesized by the citrate-reduction method and were characterized by UV-VS spectroscopic analysis, zeta-potential analysis, and size analysis. The potential of IP 18275 148 10 On: Mon 02 May 2022 14:3540 these newly generated AuNPs-CIT particles was testd on phorbol 12-myristat13-acetate (PMA)-stimulated cancer Copyrght: American Scient ic Publishers cells. Our data showed that the AuNPs-CIT were spherical, with a men size of 21.3 ?? 0.65 nm and a stabilized zetaDelivered by Ingenta potential at ???41.4 ?? 0.98 mV. These newly generated AuNPs-CIT nanoparticles inhibited PMA-induced activation and nuclear translocation of NF-KB p65 in MCF-7 cells. They also have the tendency to block TNF-a expression in stimulated cancer cells. In conclusion, AuNPs-CIT inhibits PMA-induced TNF-a mRNA and protein expression via deactivation of NF-KB signaling in breast cancer cells. These findings suggest that AuNPs-CIT might be useful in cancer treatment.