The chelating peptide (GPAGPHGPPG) derived from Alaska pollock skin enhances calcium, zinc and iron transport in Caco-2 cells

被引：45

作者：

Chen, Qianru ^{[1
]}

Guo, Lidong ^{[1
]}

Du, Fen ^{[1
]}

Chen, Tiejun ^{[1
]}

Hou, Hu ^{[1
]}

Li, Bafang ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2017年 / 52卷 / 05期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Caco-2; cell; chelating peptides; collagen; functional food; mineral transport; TANDEM MASS-SPECTROMETRY; SIMULATED GASTROINTESTINAL DIGESTION; CASEINOPHOSPHOPEPTIDE-BOUND IRON; CASEIN PHOSPHOPEPTIDES; SMALL-INTESTINE; ABSORPTION; BINDING; BIOAVAILABILITY; IDENTIFICATION; FOOD;

D O I：

10.1111/ijfs.13396

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Gly-Pro-Ala-Gly-Pro-His-Gly-Pro-Pro-Gly (GPAGPHGPPG), a chelating peptide derived from Alaska pollock skin, has been approved with outstanding ability to chelate calcium, zinc and iron ions. In this study, the stability of GPAGPHGPPG during invitro gastrointestinal enzymatic digestion, the potential binding site between peptide and metal ions as well as its effects on mineral transport in Caco-2 cells were investigated. Results showed that approximately 75% of GPAGPHGPPG remained intact at the end of the invitro gastrointestinal enzymatic digestion. The formation of peptide-metal complex was potentially related to histidine and cyclic structure in terms of dehydration. Moreover, GPAGPHGPPG revealed significant promotional effects on calcium (112.7%, P < 0.01), zinc (32.3%, P < 0.01) and iron (27.7%, P < 0.01) transport in Caco-2 cell monolayer. In conclusion, GPAGPHGPPG could be developed as a potential functional ingredient to prevent mineral deficiency.

引用

页码：1283 / 1290

页数：8

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