Separation and quantitation of sucrose esters using HPLC with evaporative light scattering detection

被引：11

作者：

Wang, Qinghui ^{[1
]}

Zhang, Shufen ^{[1
]}

Zhang, Pei ^{[1
]}

Zhu, Jinli ^{[1
]}

Yang, Jinzong ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2006年 / 29卷 / 16期

关键词：

sucrose esters; HPLC; ELSD; ESI-MS; analysis; positional isomers;

D O I：

10.1080/10826070600864874

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high performance liquid chromatographic method with evaporative light scattering detection (ELSD) for the separation and quantitation of sucrose esters is described. Samples are analyzed by means of a reversed-phase column using methanol and water under gradient conditions as the mobile phase. This procedure provides a complete separation and determination of mono- to octa-esters, with different positional isomers in each fraction on a single run, in combination with the electrospray ionization mass spectrum (ESI-MS) technology. With this method, it is possible to determine the approximate compositions of mono- to octa-esters in one analysis and the precise quantitation of pure positional isomers using an external standard method. The described method is found to be straight forward, rapid, inexpensive, and reproducible, and can be readily applied for the analysis of sucrose esters during synthesis, purification, and structure studies.

引用

页码：2399 / 2412

页数：14

共 50 条

[1] HPLC analysis of sucrose ester analogs using evaporative light scattering detection
Wang, Qinghui
Zhang, Shufen
Yang, Jinzong
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007, 30 (13-16) : 2395 - 2407
[2] Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection
Clarot, I.
Storme-Paris, I.
Chaminade, P.
Estevenon, O.
Nicolas, A.
Rieutord, A.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2009, 50 (01) : 64 - 67
[3] Separation and quantitation of cholesterol oxides by HPLC with an evaporative light scattering detector in a model system
Lakritz, L.
Jones, K.C.
JAOCS, Journal of the American Oil Chemists' Society, 1997, 74 (08): : 943 - 946
[4] Separation, identification and quantitation of phospholipid molecules by HPLC with UV, mass spectrometric and evaporative light scattering detection.
Goss, C
Johnson, BL
Le Blanc, M
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 224 : U124 - U124
[5] Separation and quantitation of cholesterol oxides by HPLC with an evaporative light scattering detector in a model system
Lakritz, L
Jones, KC
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1997, 74 (08) : 943 - 946
[6] HPLC Detection of miltefosine using an evaporative light scattering detector
Lemke, A.
Kayser, O.
PHARMAZIE, 2006, 61 (05): : 406 - 408
[7] Quantitation and profiling of orange juices and orange drinks using fast HPLC and evaporative light-scattering detection
LC GC NORTH AMERICA, 2002, : 32 - 33
[8] Simultaneous separation of nine surfactants of various types by HPLC with evaporative light scattering detection
Park, Hong Soon
Ryu, Ho Ryul
Rhee, Choong Kyun
TALANTA, 2006, 70 (03) : 481 - 484
[9] Quantitation of triacylglycerols in plant oils using HPLC with APCI-MS, evaporative light-scattering, and UV detection
Holcapek, M
Lísa, M
Jandera, P
Kabátová, N
JOURNAL OF SEPARATION SCIENCE, 2005, 28 (12) : 1315 - 1333
[10] Enantiomeric separation of an AMPA antagonist using a Chirobiotic T™ column with HPLC and evaporative light-scattering detection
Guisbert, AL
Sharp, VS
Peterson, JA
Risley, DS
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (07) : 1019 - 1028

← 1 2 3 4 5 →