Determination of furazolidone residue in eggs by high performance liquid chromatography

The determination method of furazolidone residue in eggs by high performance liquid chromatography has been developed using matrix solid-phase dispersion (MSPD) and solid-phase extraction (SPE) technologies for sample pretreatment. The retention behaviors of furazolidone on C-18 column and silica gel SPE cartridge were investigated. The analytes were quantified using external standard calibration curves. There is a good linear correlation between the peak areas and concentrations of furazolidone. The calibration coefficient exceeds 0.9999 and the dynamic linear range is 5 mug/L similar to 2.5 mg/L. The average recoveries of furazolidone in eggs ranged from 80.2% to 91.1% with the relative standard deviations between 2.0% and 9.5%, and the limit of detection is below 10 mug/kg. The relative standard deviations of intra-assay and inter-assay are both below 10%.