Presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

被引：21

作者：

Schasfoort, Richard B. M. ^{[1
]}

van Weperen, Jos ^{[2
]}

van Amsterdam, Margot ^{[1
]}

Parisot, Judicael ^{[3
]}

Hendriks, Jan ^{[4
]}

Koerselman, Michelle ^{[4
]}

Karperien, Marcel ^{[4
]}

Mentink, Anouk ^{[1
]}

Bennink, Martin ^{[5
]}

Krabbe, Hans ^{[6
,7
]}

Terstappen, Leon W. M. M. ^{[1
]}

Mulder, A. H. Leontine ^{[7
,8
]}

机构：

[1] Univ Twente, Fac Sci & Technol, Dept Med Cell BioPhys, POB 217, NL-7500 AE Enschede, Netherlands

[2] Vysens BV, POB 50035, NL-7550 LA Hengelo, Netherlands

[3] Carterra, 825 N 300 W,Suite C309, Salt Lake City, UT 84103 USA

[4] Univ Twente, Fac Sci & Technol, Dept Dev Bioengn, POB 217, NL-7500 AE Enschede, Netherlands

[5] Saxion Univ Appl Sci, NanoBio Res Grp, POB 70000, NL-7500 KB Enschede, Netherlands

[6] Med Spectrum Twente, Dept Clin Chem & Lab Med, POB 50000, NL-7500 KA Enschede, Netherlands

[7] Medlon BV, Dept Clin Chem, NL-7512 KZ Enschede, Netherlands

[8] Ziekenhuis Grp Twente, Dept Clin Chem & Lab Med, POB 7600, NL-7600 SZ Almelo, Netherlands

来源：

BIOSENSORS & BIOELECTRONICS | 2021年 / 183卷

基金：

荷兰研究理事会;

关键词：

Surface plasmon resonance imaging; SPR; Affinity; off-rate; Biosensor; CORONAVIRUS;

D O I：

10.1016/j.bios.2021.113165

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 119 CoViD-19 patients. The SPRi assay measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 and this new method can furthermore be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 in vaccination programs.

引用

页数：4

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