Selective detection of Cys and GSH by using one fluorescent probe at two excitation wavelengths

被引：5

作者：

Xiao, Yintang ^{[1
]}

Guo, Kai ^{[1
]}

Wei, Jun ^{[1
]}

Gao, Xiaowei ^{[1
]}

Yi, Dong ^{[1
]}

Li, Yang ^{[1
]}

Yu, Xiaoqi ^{[2
]}

Zhang, Chun ^{[1
]}

Wang, Qin ^{[1
]}

机构：

[1] Southwest Med Univ, Sch Pharm, Key Lab Med Electrophysiol, Minist Educ, Luzhou 646000, Peoples R China

[2] Sichuan Univ, Coll Chem, Key Lab Green Chem & Technol, Minist Educ, Chengdu 610064, Peoples R China

来源：

TETRAHEDRON LETTERS | 2020年 / 61卷 / 44期

关键词：

Selective detection; Fluorescent probe; Amino acids; Mechanistic study; Cell imaging; CYSTEINE; DISCRIMINATION; GLUTATHIONE;

D O I：

10.1016/j.tetlet.2020.152462

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Selective detection of Cys and GSH by using one fluorescent probe with two different excitation wavelengths is developed based on a naphthyl-coumarin aldehyde probe. Mechanistic studies indicate that the distinctively different fluorescent responses of the probe toward Cys and GSH at the two excitation wavelengths are due to two different reaction pathways that generate different products. The probe has been used to detect Cys and GSH in HeLa cells by using two emission channels. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：4

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