Molecular characterization of 26S proteasome regulatory subunit in dermatophyte pathogen Trichophyton verrucosum

被引：0
|
作者：
Fouladvand, Moradali [1 ]
Naeimi, Behrouz [1 ,2 ]
Rezaie, Sassan [3 ]
Noorbakhsh, Fatemeh [4 ]
Safari, Mohammad Reza [3 ]
机构：
[1] Bushehr Univ Med Sci, Fac Med, Dept Microbiol & Parasitol, Bushehr, Iran
[2] Bushehr Univ Med Sci, Persian Gulf Trop & Infect Dis Res Ctr, Bushehr, Iran
[3] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Mycol & Parasitol, Div Mol Biol, Tehran, Iran
[4] Islamic Azad Univ, Varamin Pishva Branch, Varamin, Iran
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 12期
关键词：
Dermatophyte; Trichophyton verrucosum; fungal DNA; nucleic acid sequencing; 26S proteasome; 20S PROTEASOME; IDENTIFICATION; PROTEIN;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Trichophyton verrucosum is a zoophilic dermatophyte, which causes dermatophytosis infection in human as well as animals. 26S proteasome is an important protein in eukaryotic cells that is involved with degradation of unneeded or damaged proteins, when tagged with ubiquitin. In this study, we characterized the 26S Proteasome regulatory subunit gene in dermatophyte pathogen T. verrucosum. High molecular weight DNA has been isolated from T. verrucosum and utilized with pairs of specific nucleotides primers, designed from highly preserved regions of the 26S proteasome regulatory subunit genes. Obtained DNA fragments were sequenced and the results were analyzed in GenBank. This DNA fragment, which contains no intron within its open reading frame, encodes a polypeptide with 332 amino acids. The characterized PCR fragments revealed significant homology with other 26S proteasome regulatory subunit genes in GenBank.
引用
收藏
页码：2695 / 2699
页数：5
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