Stability of stereoregular oligo(nucleoside phosphorothioate)s in human cells. Diastereoselectivity of cellular 3'-exonuclease

被引：3
|
作者：
Koziolkiewicz, M
Gendaszewska, E
Maszewska, M
机构：
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1997年 / 16卷 / 7-9期
关键词：
D O I：
10.1080/07328319708006254
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Stability of oligo(nucleoside phosphorothioate)s (PS-oligos) in HUVEC (human umbilical vein endothelial cells) has been studied. Cytosolic fraction of HUVEC possesses 3'-exonucleolytic activity which is responsible for degradation of natural oligomers and PS-oligos. The enzyme is R-P-specific, i.e. it cleaves internucleotide phosphorothioate function of R-P- and not S-P-configuration at phosphorus atom.
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页码：1677 / 1682
页数：6
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