Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae

被引：5
|
作者：
Sugiyama, K
Niki, TP
Inokuchi, K
Teranishi, Y
Ueda, M
Tanaka, A
机构：
[1] GenCom, Machida, Tokyo 1948511, Japan
[2] Mitsubishi Kagaku Inst Life Sci, Machida, Tokyo 1948511, Japan
[3] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Lab Appl Biol Chem,Sakyo Ku, Kyoto 6068501, Japan
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2004年 / 64卷 / 04期
关键词：
D O I：
10.1007/s00253-003-1544-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae. The production of rat metabotropic glutamate receptor 1alpha (mGluR1alpha), which belongs to the G-protein-coupled receptor (GPCR) family, was tested under the control of UPR-ICL. Expression of mGluR1alpha was found in recombinant clones and enhanced by replacing the signal sequence of mGluR1alpha with the corresponding region of the alpha-factor receptor (Ste2), which is a GPCR found in S. cerevisiae. Moreover, the membrane fraction from a recombinant clone associated with Vesl-1S/Homer-1a protein binds the mGluR1alpha in rat cerebellum. These results suggest that the UPR-ICL-controlled gene expression system is useful for heterologous GPCRs in S. cerevisiae.
引用
收藏
页码：531 / 536
页数：6
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