The upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae. The production of rat metabotropic glutamate receptor 1alpha (mGluR1alpha), which belongs to the G-protein-coupled receptor (GPCR) family, was tested under the control of UPR-ICL. Expression of mGluR1alpha was found in recombinant clones and enhanced by replacing the signal sequence of mGluR1alpha with the corresponding region of the alpha-factor receptor (Ste2), which is a GPCR found in S. cerevisiae. Moreover, the membrane fraction from a recombinant clone associated with Vesl-1S/Homer-1a protein binds the mGluR1alpha in rat cerebellum. These results suggest that the UPR-ICL-controlled gene expression system is useful for heterologous GPCRs in S. cerevisiae.
机构:
Univ South Dakota, Sanford Sch Med, Sioux Falls, SD USAUniv South Dakota, Sanford Sch Med, Sioux Falls, SD USA
Hof, Caitlin
Burris, Larry
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Univ South Dakota, Sanford Sch Med, Internal Med, Sioux Falls, SD USA
Sanford Med Ctr, Neurocrit Care, Sioux Falls, SD USAUniv South Dakota, Sanford Sch Med, Sioux Falls, SD USA
机构:
Zhejiang University of Science and Technology, Hangzhou
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, HangzhouZhejiang University of Science and Technology, Hangzhou
Xue C.
Zhang P.
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Zhejiang University of Science and Technology, Hangzhou
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, HangzhouZhejiang University of Science and Technology, Hangzhou
Zhang P.
Bai L.
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Zhejiang University of Science and Technology, Hangzhou
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, HangzhouZhejiang University of Science and Technology, Hangzhou
Bai L.
Xiao G.
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Zhejiang University of Science and Technology, Hangzhou
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, HangzhouZhejiang University of Science and Technology, Hangzhou
Xiao G.
Wei P.
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Zhejiang University of Science and Technology, Hangzhou
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, HangzhouZhejiang University of Science and Technology, Hangzhou
机构:
Univ Calif Berkeley, Lawrence Berkeley Lab, Donner Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Lab, Donner Lab, Div Life Sci, Berkeley, CA 94720 USA
Andersen, B
Stevens, RC
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机构:Univ Calif Berkeley, Lawrence Berkeley Lab, Donner Lab, Div Life Sci, Berkeley, CA 94720 USA