Identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma

被引：8
|
作者：
Fujino, Yasuhito [1 ,2 ]
Liao, Chun-Peng [3 ]
Zhao, Yan Shi [2 ]
Pan, Judong [2 ]
Mathes, Lawrence E. [4 ,5 ]
Hayes, Kathleen A. [4 ,5 ]
Ohno, Koichi [1 ]
Tsujimoto, Hajime [1 ]
Roy-Burman, Pradip [2 ,3 ]
机构：
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, Tokyo 1138657, Japan
[2] Univ So Calif, Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
[3] Univ So Calif, Sch Med, Dept Biochem & Mol Biol, Los Angeles, CA 90033 USA
[4] Ohio State Univ, Ctr Retrovirus Res, Columbus, OH 43210 USA
[5] Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA
来源：
VIROLOGY | 2009年 / 386卷 / 01期
关键词：
Retrovirus; Insertional mutagenesis; ACVRL1/ALK1; RETROVIRAL INSERTIONAL MUTAGENESIS; C-MYC; CELL-LINE; GENE; CONSERVATION; ACTIVIN; FLVI-2; TUMORS; LYMPHOSARCOMAS; EXPRESSION;
D O I：
10.1016/j.virol.2009.01.021
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new proviral integration site for feline leukemia virus (FeLV), termed flit-1, was identified from feline thymic lymphoma. Among 35 FeLV-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one T-lymphoid leukemia examined had rearrangement in this region. Extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromosome 15. The human and murine homologs of flit-1 are positioned approximately 30-kb upstream to activin-A receptor type II-like 1 (ACVRL1/ALK1) gene. Expression of ACVRL1 mRNA was examined in two of five lymphomas with flit-1 rearrangement and detected in both of the two whereas normal thymuses and seven lymphoid tumors without flit-1 rearrangement had no detectable expression. Therefore, flit-1 appears to represent a novel FeLV proviral common integration domain that may influence lymphomagenesis as insertional mutagenesis. (c) 2009 Elsevier Inc. All rights reserved.
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页码：16 / 22
页数：7
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