Characterization of pearl millet prolamins

被引：10
|
作者：
Marcellino, LH [1 ]
Junior, CB [1 ]
Gander, ES [1 ]
机构：
[1] EMBRAPA, Cenargen, Brasilia, DF, Brazil
来源：
PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 03期
关键词：
cereals; Pennisetum glaucum; seed storage proteins; prolamins; pennisetin;
D O I：
10.2174/0929866023408724
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF/MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values around 27, 22 and 12 kDa, respectively, We present partial amino acid sequences of these major proteins, which should allow the posterior isolation of the respective genes.
引用
收藏
页码：237 / 244
页数：8
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