Nucleic acid in situ probing - Hybridization to human chromosomes of an alkaline phosphatase labeled centromeric probe

被引：1
|
作者：
Nardone, RM [1 ]
机构：
[1] CATHOLIC UNIV AMER,CTR ADV TRAINING CELL MOL BIOL,WASHINGTON,DC 20064
来源：
MOLECULAR BIOTECHNOLOGY | 1997年 / 7卷 / 02期
关键词：
in situ hybridization; centromere; chromosomes; nucleic acid probes;
D O I：
10.1007/BF02761752
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The main principles that underly the use of nucleic acid probes for in situ hybridization are summarized. These include probe design, target preparation, hybridization formats and conditions, and signal generating systems. These principles underly the specific protocol that is described, namely the use of an akaline phosphatase-labeled cloned sequence of the alphoid repeated DNA family as a centomere probe for human chromosomes.
引用
收藏
页码：165 / 172
页数：8
相关论文
共 50 条
12345