Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria

被引：14
|
作者：
Nagayoshi, Chizuru [1 ]
Ishibashi, Matsujiro [1 ]
Tokunaga, Masao [1 ]
机构：
[1] Kagoshima Univ, Fac Agr, Kagoshima 8900065, Japan
来源：
PROTEIN AND PEPTIDE LETTERS | 2009年 / 16卷 / 02期
关键词：
Human serine racemase; porin; groE; His-tag; halophile; ESCHERICHIA-COLI; MESSENGER-RNA; RAT-BRAIN; RECEPTOR; PROTEIN; RELEASE; NEURONS;
D O I：
10.2174/092986609787316261
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
引用
收藏
页码：201 / 206
页数：6
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