Applications of Total Internal Reflection Fluorescence (TIRF) Microscopy in Cellular Bio-imaging

被引:0
|
作者
Jin, L. [1 ,2 ]
Lee, R. K. [3 ]
Kong, S. K. [3 ]
Yuan, W. [1 ,2 ]
Ho, H. P. [1 ,2 ]
Lin, Chinlon [1 ,2 ]
机构
[1] Chinese Univ Hong Kong, Ctr Adv Res Photon, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Dept Elect Engn, Shatin, Hong Kong, Peoples R China
[3] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China
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中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Evanescent field based imaging and sensor technologies have been widely used for a long time, but only recently has TIRF been introduced for bio-imaging. This paper will discuss the reasons and give a brief summary of the recent development of TIRF which is also based on evanescent wave excitation, and which is well suited for ultra thin optical sectioning (hundreds of nanometres) at cell-substrate regions with an unusually high signal-to-noise ratio and image quality.
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页码:587 / +
页数:2
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