Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold

被引:159
|
作者
Chen, Xiaoqing [1 ]
Zhou, Guobao [1 ,2 ]
Song, Ping [2 ]
Wang, Jingjing [2 ,3 ]
Gao, Jimin [3 ]
Lu, Jianxin [3 ]
Fan, Chunhai [2 ]
Zuo, Xiaolei [2 ]
机构
[1] Cent S Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Resource Conserving & Envi, Changsha 410083, Hunan, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China
[3] Wenzhou Med Univ, Sch Med Lab Sci & Life Sci, Wenzhou 325035, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
PROMISING PLASMA BIOMARKERS; REDEFINES UNDETECTABLE PSA; ASSAY REVEALS PROSTASOMES; BIOCHEMICAL RECURRENCE; ELECTRON-TRANSFER; BINDING; IMMOBILIZATION; CONSTRUCTION; SURFACES;
D O I
10.1021/ac500054x
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The high occurrence of prostate cancer in men makes the prostate-specific antigen (PSA) screening test really important. More importantly, the recurrence rate after radical prostatectomy is high, whereas the traditional P SA immunoassay does not possess the sufficient high sensitivity for post-treatment PSA detection. In these assays, uncontrolled and random orientation of capture antibodies on the surface largely reduces their activity. Here, by exploiting the rapidly emerging DNA nanotechnology, we developed a DNA nanostructure based scaffold to precisely control the assembly of antibody monolayer. We demonstrated that the detection sensitivity was critically dependent on the nanoscale-spacing (nanospacing) of immobilized antibodies. In addition to the controlled assembly, we further amplified the sensing signal by using the gold nanoparticles, resulting in extremely high sensitivity and a low detection limit of 1 pg/mL. To test the real-world applicability of our nanoengineered electrochemical sensor, we evaluated the performance with 11 patients' serum samples and obtained consistent results with the "gold-standard" assays.
引用
收藏
页码:7337 / 7342
页数:6
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