Development and Application of PPA-ELISA for Detecting Antibodies to Classical Swine Fever Virus Using Recombinant E2 Antigen

被引：0

作者：

Li Jiao ^{[1
]}

Wang Jin-liang ^{[2
]}

Zu Li-chuang ^{[1
]}

Xie Jin-wen ^{[2
]}

Shen Zhi-qiang ^{[1
,2
]}

机构：

[1] Shandong Lvdu Biosci & Technol Co Ltd, Binzhou 256600, Peoples R China

[2] Shandong Binzhou Anim Sci & Vet Med Acad, Binzhou 256600, Peoples R China

来源：

ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 2 | 2011年

关键词：

classical swine fever virus; recombinant E2 protein antigen; PPA-ELISA; diagnosis; GLYCOPROTEIN E2; VACCINE;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

To develop a method to detect the antibody of classical swine fever virus(CSFV), An indirect ELISA kit was developed using the purified recombinant E2 protein as coating antigen and HorseradiSh peroxidase-staplylococcal protein(HRP-SPA) as the second antibody. This E2-PPA-ELISA Kit has good specificity, sensitivity and repeatability, could be used as a simple and rapid serology detection method for monitoring of anti-CSFV antibody and epidemiologic survey of CSFV.

引用

页码：93 / 96

页数：4

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