Multiplex RT-PCR for Rapid Detection of Viruses Commonly Causing Diarrhea in Pediatric Patients

被引：45

作者：

Thongprachum, Aksara ^{[1
,2
]}

Khamrin, Pattara ^{[3
]}

Ngan Thi Kim Pham ^{[1
]}

Takanashi, Sayaka ^{[2
]}

Okitsu, Shoko ^{[1
,2
]}

Shimizu, Hiroyuki ^{[4
]}

Maneekarn, Niwat ^{[3
]}

Hayakawa, Satoshi ^{[1
]}

Ushijima, Hiroshi ^{[1
,2
]}

机构：

[1] Nihon Univ, Sch Med, Dept Pathol & Microbiol, Div Microbiol, Tokyo, Japan

[2] Univ Tokyo, Sch Int Hlth, Grad Sch Med, Dept Dev Med Sci, Tokyo, Japan

[3] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai, Thailand

[4] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan

来源：

JOURNAL OF MEDICAL VIROLOGY | 2017年 / 89卷 / 05期

基金：

日本学术振兴会;

关键词：

multiplex RT-PCR; pediatric patients; diarrheal viruses; AICHI VIRUS; ENTEROVIRUSES; ASTROVIRUS; CHILDREN; SAMPLES;

D O I：

10.1002/jmv.24711

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. (C) 2016 Wiley Periodicals, Inc.

引用

页码：818 / 824

页数：7

共 50 条

[1] A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum
Song, A.
You, Y.
Chen, F.
Li, P.
Jiang, J.
Chen, S.
LETTERS IN APPLIED MICROBIOLOGY, 2013, 56 (01) : 8 - 13
[2] A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine
Zhao, Yan
Liu, Feifei
Li, Qingmei
Wu, Mengfan
Lei, Lei
Pan, Zishu
JOURNAL OF VIROLOGICAL METHODS, 2019, 269 : 38 - 42
[3] Detection of respiratory viruses by multiplex RT-PCR with a GeXP analyzer
Lu, X.
Wang, Q.
Zhang, Y.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2014, 21 : 444 - 444
[4] Multiplex nested RT-PCR for the detection of porcine enteric viruses
Ben Salem, Abid Nabil
Sergei, A. Chupin
Olga, P. Bjadovskaya
Olga, G. Andreeva
Mahjoub, Aouni
Larissa, B. Prokhvatilova
JOURNAL OF VIROLOGICAL METHODS, 2010, 165 (02) : 283 - 293
[5] A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea
Khamrin, Pattara
Okame, Makiko
Thongprachum, Aksara
Nantachit, Nattika
Nishimura, Shuichi
Okitsu, Shoko
Maneekarn, Niwat
Ushijima, Hiroshi
JOURNAL OF VIROLOGICAL METHODS, 2011, 173 (02) : 390 - 393
[6] POCT Detection of 14 Respiratory Viruses Using Multiplex RT-PCR
Lee, Ji Su
Ahn, Jeong Jin
Kim, Seung Jun
Yu, So Yeon
Koh, Eun Jung
Kim, Seung Hwan
Sung, Heung Sup
Huh, Jin Won
Hwang, Seung Yong
BIOCHIP JOURNAL, 2021, 15 (04) : 371 - 380
[7] Multiplex RT-PCR detection of three common viruses infecting orchids
Raymond N. Ali
Alison L. Dann
Peter A. Cross
Calum R. Wilson
Archives of Virology, 2014, 159 : 3095 - 3099
[8] POCT Detection of 14 Respiratory Viruses Using Multiplex RT-PCR
Ji Su Lee
Jeong Jin Ahn
Seung Jun Kim
So Yeon Yu
Eun Jung Koh
Seung Hwan Kim
Heung Sup Sung
Jin Won Huh
Seung Yong Hwang
BioChip Journal, 2021, 15 : 371 - 380
[9] Multiplex RT-PCR detection of three common viruses infecting orchids
Ali, Raymond N.
Dann, Alison L.
Cross, Peter A.
Wilson, Calum R.
ARCHIVES OF VIROLOGY, 2014, 159 (11) : 3095 - 3099
[10] Multiplex RT-PCR detection and microarray typing of vesicular disease viruses
Lung, Oliver
Fisher, Mathew
Beeston, Anne
Hughes, Kimberley Burton
Clavijo, Alfonso
Goolia, Melissa
Pasick, John
Mauro, William
Deregt, Dirk
JOURNAL OF VIROLOGICAL METHODS, 2011, 175 (02) : 236 - 245

← 1 2 3 4 5 →