Expressions of miR-193b and miR-338-3p in renal clear-cell carcinoma cells and their effects on biological function

Objective: To evaluate the expressions of miR-193b and miR-338-3p in renal clear-cell carcinoma cells and their effects on biological function. Methods: Cancer tissues, adjacent normal tissues, and serum samples were obtained from 34 patients with renal clear-cell carcinoma. Peripheral blood samples from 30 healthy subjects were used as controls. The expression levels of miR-193b and miR-338-3p in renal clear-cell carcinoma cells and serums and their effects on the proliferation and invasion of renal clear-cell carcinoma cells were analyzed. Results: The relative expression level of miR-193b was significantly higher in cancer tissues of patients with renal clear-cell carcinoma than in the adjacent noncancerous tissues (P<0.05). The relative expression of miR-338-3p was significantly lower in the cancer tissue than in the adjacent noncancerous tissues (P<0.05). The relative expression level of miR-193b in the serum of patients was significantly higher than that observed in the serum of healthy subjects (P<0.05). The relative expression level of miR-338-3p was significantly lower in patients than in healthy subjects (P<0.05). The results of MTT in vitro proliferation test showed statistical differences in the absorbance values of the three groups treated with miR-193b mimic at each time point (P<0.05). Transwell in vitro invasion test showed statistically significant differences in the number of invading cells in the three groups subjected to miR-193b mimic treatment (P<0.05). Significant difference was also observed in the number of invading cells between miR-193b mimic group (higher) and miR-control group (P<0.05). There were statistical differences in the number of transmembrane cells in the three groups of miR-338-3p cells (P<0.05). The number of transmembrane cells of miR-338-3p mimic and miR-control group was significantly lower than that of miR-338-3p inhibitor group (P<0.05), and the number of transmembrane cells of miR-338-3p mimic group significantly lower than the miR-control group (P<0.05). Conclusion: miR-193b and miR-338-3p was associated with renal clear-cell carcinoma. miR-193b has a function similar to that of a tumor inducer gene, while miR-338-3p functions as a tumor suppressor.